Friday, December 6, 2024
Home Neuroscience New technique to freeze brain tissue without harm

New technique to freeze brain tissue without harm

by Medical Xpress
0 comment


Credit: Cell Reports Methods (2024). DOI: 10.1016/j.crmeth.2024.100777

A team of medical researchers at the National Children’s Medical Center, Children’s Hospital, Fudan University, in China, has developed a technique to freeze and thaw brain tissue without causing damage.

In their study, published in the journal Cell Reports Methods, the group tested bathing brain tissue in candidate chemicals before freezing them using .

Prior research has shown that no matter how quickly brain matter is frozen, the freezing and thawing process always causes . This has made it more difficult for researchers to study brain matter because research must be conducted immediately after obtaining a . In this new effort, the team in China found a way around this problem by soaking the tissue in a special solution before freezing.

The work involved dipping or soaking brain organoids (brain tissue grown from ) in candidate compounds and then freezing and thawing them to see how they fared. After many attempts, they found one combination of solutions that worked best—a mix of ethylene glycol, methylcellulose DMSO and Y27632. They named the solution mix MEDY.

The research team then tested MEDY under a variety of conditions to see how well it prevented damage from freezing. The conditions involved changing variables, such as the age of the organoids prior to freezing and how long they were soaked in a MEDY solution. They then allowed the organoids to resume growing after they were thawed for up to 150 days.

The researchers found little difference between organoids that had been frozen and those that had not—even those that had been frozen for as long as 18 months.

As a final test, the research team used their technique on a sample of brain tissue obtained from a live human patient and found that it worked just as well.

The research team suggests that their technique should allow researchers to store samples on a scale large enough to allow for new types of brain and nervous system research.

More information:
Weiwei Xue et al, Effective cryopreservation of human brain tissue and neural organoids, Cell Reports Methods (2024). DOI: 10.1016/j.crmeth.2024.100777

© 2024 Science X Network

Citation:
New technique to freeze brain tissue without harm (2024, May 17)
retrieved 17 May 2024
from https://medicalxpress.com/news/2024-05-technique-brain-tissue.html

This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no
part may be reproduced without the written permission. The content is provided for information purposes only.





Source link

You may also like

Leave a Comment

Edtior's Picks

Latest Articles

All Rights reserved, site designed by Yellohost.co.za